This article has an erratum: [https://doi.org/10.1051/mbcb/2023015]
J Oral Med Oral Surg
Volume 29, Number 1, 2023
|Number of page(s)||11|
|Published online||03 February 2023|
Original Research Article
Evaluation of differential white blood cell count and cheek pouch epithelium in 7,12-dimethylbenza[a]anthracene hamster carcinogenesis model, managed with three phytochemicals
Oral Medicine and Periodontology Department, Faculty of Dentistry, Mansoura University, Mansoura, Egypt
2 Oral Pathology Department, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt
3 Department of Oncology, Faculty of Medicine, McGill University, Montreal, Quebec, Canada
* Correspondence: email@example.com
Accepted: 5 January 2023
Objectives: Nigella sativa (NS), thymoquinone (TQ), and epigallocatechin-3-gallate (EGCG) are phytochemicals that might have antioxidant protective potentials on the hamster cheek pouch epithelium (HCPE). We aimed at evaluating and comparing the potential therapeutic outcomes of these 3 phytochemicals by analysis of peripheral white blood cells (WBCs) counts. Materials and Methods: NS whole oil, TQ and EGCG were administered before, with or after 7,12-dimethylbenza[a]anthracene (DMBA) painting the hamster left cheek pouch. Before sacrificing each animal, 2 ml of blood was withdrawn into a fine heparin-containing tube to estimate the total WBCs, lymphocytes, MID cells, and granulocytes counts by an automatic count system. All cheek pouches were surgically excised and examined with light microscope. Results: Severe epithelial dysplasia was evident after 6 weeks of DMBA administration, and when NS was given for 2 weeks followed by DMBA for 6 weeks. When NS or EGCG were given for 2 weeks then continued with DMBA for 6 weeks, mild dysplasia was seen. When DMBA was given for 6 weeks followed by NS or TQ for 6 weeks, mild dysplasia was noted. Administration of DMBA for 6 weeks resulted in significant reduction in total WBCs and lymphocytes counts compared to healthy controls. Administration of NS or TQ for 2 weeks resulted in significant elevation in lymphocytes count compared to healthy controls. Significant elevation in total WBCS and lymphocytes counts was noted when EGCG was given for 2 weeks and continued with DMBA for other 6 weeks. Similar results were noted when DMBA was given for 6 weeks followed by TQ for 6 weeks when compared to NS, DMBA or healthy controls. Discussion: The three phytochemicals showed different levels of protection against DMBA carcinogenic activity, more specifically, TQ and NS had higher therapeutic potential and might be used for treatment and/or preventive management of oral cancer in the future. Conclusion: However, further investigations are required to address the mechanism of action and feasibility of clinical application of each phytochemical.
Key words: Epigallocatechin-3- gallate / HCPE-DMBA / Nigella sativa / thymoquinone / peripheral WBCs / phytochemicals
Note to the reader: The co-author's names "Mahmoud H. Darwish and Magda M. Hassan" were incorrect. Following the publication of the Correction Notice, it has been corrected to "Mahmoud Darwish and Magda Hassan" on April 7, 2023.
© The authors, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.