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Table II

Methods and protocols for PC preparations.

Platelet concentrates Preparation
PRP [7] PRP Method:
Blood collected in sterile tubes containing anti-coagulant.
First Spin: ‘Soft Spin’,
After separation of erythrocytes,
Second Spin: ‘Hard Spin’, PRP should be separated from platelet poor plasma (PPP).
Buffy Coat Method:
Blood should be collected and stored at 20−24°C
First Spin: High Speed
PPP formed at the top should be removed and buffy coat layer (WBC and Platelets) should be transferred into another tube.
Second Spin: Low speed to separate WBC's
L-PRF or S-PRF [12] 2700 rpm for 12 min
A-PRF [12] 1500 rpm for 14 min
A-PRF + [10] 1300 rpm for 8 min
i-PRF [13] 700 rpm for 3 min

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